Source code for ewoksndreg.tasks.reg2d_intensities

from typing import Any
from typing import Dict
from typing import List
from typing import Optional
from typing import Union

import numpy
from ewokscore.model import BaseInputModel
from ewokscore.model import BaseOutputModel
from ewokscore.task import Task
from pydantic import Field

from ..intensities import registration
from ..io.input_stack import InputDataType
from ..io.input_stack import input_context
from ..registry import RegistryIdType
from ..transformation.base import Transformation
from ..transformation.types import TransformationType


[docs] class Inputs(BaseInputModel): image_stacks: InputDataType = Field( description="Image stacks as a dictionary of numpy arrays or list of HDF5 dataset URI's.", examples=[ { "stack1": "/path/to/file.h5::/entry/process/results/parameters/Ca-K", "stack2": "/path/to/file.h5::/entry/process/results/parameters/Fe-K", }, {"stack1": [[0, 0, 0], [1, 1, 1], [2, 2, 2]]}, ], ) mapper: RegistryIdType = Field( description="Method to find parameters of the transformation between the image intensities.", examples=["LstSq-Numpy", "LstSq-SciPy", "Ransac-SciKitImage"], ) transformation_type: TransformationType = Field( description="Type of transformation between the intensities.", examples=["Translation", "Rigid", "Affine"], ) reference_image: Union[int, float] = Field( default=0, description="The index of the reference image in the stack (0.5 is the middle of the stack)." "The calculated transformations will be relative to this image.", examples=[0, -1, 0.5], ) reference_stack: Optional[str] = Field( default=None, description="Transformations of all stacks is based on the image registration of this stack.", examples=["stack1", "stack2"], ) block_size: int = Field( default=1, description="Register images within blocks and then register with respect to the reference. " "Pair-wise registration can be done with `block_size=2`." "Useful when images drift alot over the entire stack.", examples=[2, 5], ) mask: Optional[numpy.ndarray] = Field( default=None, description="Boolean image mask applied to the image before calculating the transformation (False means masked-off).", examples=[[[True, True, True], [True, True, True], [False, True, True]]], ) preprocessing_options: Optional[dict] = Field( default=None, description="Filters, windows and other operations that will be applied to the image before calculating the transformation.", examples=[{"apply_filter": "median"}], ) mapper_options: Optional[dict] = Field( default=None, description="Method dependent parameters.", examples=None, ) output_configuration: Optional[Dict[str, Any]] = Field( default=None, description="Registration configuration parameters to be saved.", examples=[{"param1": 0, "param2": 1}], )
[docs] class Outputs(BaseOutputModel): image_stacks: InputDataType = Field( description="Dictionary of image stack as numpy arrays." ) transformations: Dict[str, List[Transformation]] = Field( description="Transformation between the images of each stack." ) reference_stack: Optional[str] = Field( description="Transformations of all stacks is based on the image registration of this stack.", examples=["stack1", "stack2"], ) output_configuration: Optional[Dict[str, Any]] = Field( description="Registration configuration parameters to be saved.", examples=[{"param1": 0, "param2": 1}], )
[docs] class Reg2DIntensities(Task, input_model=Inputs, output_model=Outputs): """Use an intensity-based registration method to calculate transformations between the images in one or more stacks."""
[docs] def run(self): mapper_options = self.inputs.mapper_options or dict() mapper = registration.IntensityMapping.get_subclass(self.inputs.mapper)( transfo_type=self.inputs.transformation_type, mask=self.inputs.mask, **mapper_options, ) stacks_to_align = self.inputs.image_stacks reference_stack = self.inputs.reference_stack if reference_stack: if reference_stack not in stacks_to_align: raise ValueError( f"{reference_stack=} must be in {list(stacks_to_align)}" ) stacks_to_align = {reference_stack: stacks_to_align[reference_stack]} with input_context(stacks_to_align) as stacks: transformations = registration.calculate_transformations( stacks, mapper, reference_image=self.inputs.reference_image, block_size=self.inputs.block_size, preprocessing_options=self.inputs.preprocessing_options, ) if reference_stack: names = list(self.inputs.image_stacks) transformations = {name: transformations[reference_stack] for name in names} self.outputs.transformations = transformations self.outputs.reference_stack = reference_stack self.outputs.image_stacks = self.inputs.image_stacks output_configuration = self.get_input_value("output_configuration") or dict() output_configuration["mapper"] = str(mapper.get_subclass_id()) output_configuration["mapper_options"] = mapper_options output_configuration["transformation_type"] = mapper.transformation_type.name output_configuration["reference_image"] = self.inputs.reference_image output_configuration["reference_stack"] = reference_stack output_configuration["preprocessing_options"] = ( self.inputs.preprocessing_options ) output_configuration["block_size"] = self.inputs.block_size self.outputs.output_configuration = output_configuration