Source code for ewoksndreg.tasks.reg2d_intensities

from typing import Optional
from typing import Union

import numpy
from ewokscore.model import BaseInputModel
from ewokscore.task import Task
from pydantic import Field

from ..intensities import registration
from ..io.input_stack import InputDataType
from ..io.input_stack import input_context
from ..registry import RegistryIdType
from ..transformation.types import TransformationType


[docs] class Inputs(BaseInputModel): image_stacks: InputDataType = Field( ..., description="Image stacks as a dictionary of numpy arrays or list of HDF5 dataset URI's.", examples=[ { "stack1": "/path/to/file.h5::/entry/process/results/parameters/Ca-K", "stack2": "/path/to/file.h5::/entry/process/results/parameters/Fe-K", }, {"stack1": [[0, 0, 0], [1, 1, 1], [2, 2, 2]]}, ], ) mapper: RegistryIdType = Field( ..., description="Method to find parameters of the transformation between the image intensities.", examples=["LstSq-Numpy", "LstSq-SciPy", "Ransac-SciKitImage"], ) transformation_type: TransformationType = Field( ..., description="Type of transformation between the intensities.", examples=["Translation", "Rigid", "Affine"], ) reference_image: Union[int, float] = Field( 0, description="The index of the reference image in the stack (0.5 is the middle of the stack)." "The calculated transformations will be relative to this image.", examples=[0, -1, 0.5], ) reference_stack: Optional[str] = Field( None, description="Transformations of all stacks is based on the image registration of this stack.", examples=["stack1", "stack2"], ) block_size: int = Field( 1, description="Register images within blocks and then register with respect to the reference. " "Pair-wise registration can be done with `block_size=2`." "Useful when images drift alot over the entire stack.", examples=[2, 5], ) mask: Optional[numpy.ndarray] = Field( None, description="Boolean image mask applied to the image before calculating the transformation (False means masked-off).", examples=[[[True, True, True], [True, True, True], [False, True, True]]], ) preprocessing_options: Optional[dict] = Field( None, description="Filters, windows and other operations that will be applied to the image before calculating the transformation.", examples=[{"apply_filter": "median"}], ) mapper_options: Optional[dict] = Field( None, description="Method dependent parameters.", examples=None, ) output_configuration: Optional[dict] = Field( None, description="Registration configuration parameters to be saved.", examples=[{"param1": 0, "param2": 1}], )
[docs] class Reg2DIntensities( Task, input_model=Inputs, output_names=[ "image_stacks", "transformations", "reference_stack", "output_configuration", ], ): """Use an intensity-based registration method to calculate transformations between the images in one or more stacks."""
[docs] def run(self): mapper_options = self.inputs.mapper_options or dict() mapper = registration.IntensityMapping.get_subclass(self.inputs.mapper)( transfo_type=self.inputs.transformation_type, mask=self.inputs.mask, **mapper_options, ) stacks_to_align = self.inputs.image_stacks reference_stack = self.inputs.reference_stack if reference_stack: if reference_stack not in stacks_to_align: raise ValueError( f"{reference_stack=} must be in {list(stacks_to_align)}" ) stacks_to_align = {reference_stack: stacks_to_align[reference_stack]} with input_context(stacks_to_align) as stacks: transformations = registration.calculate_transformations( stacks, mapper, reference_image=self.inputs.reference_image, block_size=self.inputs.block_size, preprocessing_options=self.inputs.preprocessing_options, ) if reference_stack: names = list(self.inputs.image_stacks) transformations = {name: transformations[reference_stack] for name in names} self.outputs.transformations = transformations self.outputs.reference_stack = reference_stack self.outputs.image_stacks = self.inputs.image_stacks output_configuration = self.get_input_value("output_configuration") or dict() output_configuration["mapper"] = str(mapper.get_subclass_id()) output_configuration["mapper_options"] = mapper_options output_configuration["transformation_type"] = mapper.transformation_type.name output_configuration["reference_image"] = self.inputs.reference_image output_configuration["reference_stack"] = reference_stack output_configuration["preprocessing_options"] = ( self.inputs.preprocessing_options ) output_configuration["block_size"] = self.inputs.block_size self.outputs.output_configuration = output_configuration